New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica

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New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica

BACKGROUND The oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the production of recombinant proteins. At present, several promoters with different strengths have been developed based either on the constitutive pTEF promoter or on oleic acid inducible promoters such as pPOX2 and pLIP2. Although these promoters are highly efficient, there is still a lack...

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Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.

The development of strong and tunable promoter elements is necessary to enable metabolic and pathway engineering applications for any host organism. Here, we have expanded and generalized a hybrid promoter approach to produce libraries of high-expressing, tunable promoters in the nonconventional yeast Yarrowia lipolytica. These synthetic promoters are comprised of two modular components: the en...

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Protein expression and secretion in the yeast Yarrowia lipolytica.

Strains and vectors for protein expression and secretion have been developed in the yeast Yarrowia lipolytica. Host strains were constructed with non-reverting auxotrophic markers, deletions of protease-encoding genes, and carrying a docking platform. To drive transcription, either the synthetic hp4d or the inducible POX2 promoter were used. Protein secretion is either directed by the targeting...

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Expression Pattern of the Synthetic Pathogen-Inducible Promoter (SynP-FF) in the Transgenic Canola in Response to Sclerotinia sclerotiorum

Sclerotinia sclerotiorum is a phytopathogenic fungus which causes serious yield losses in canola. A pathogen inducible-promoter can facilitate the production of Sclerotinia-resistant transgenic canola plants. Inthis study, the “gain of function approach” was adopted for the construction of a pathogen-inducible promoter.The synthetic promoter technique was used, which involved the in...

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Yarrowia lipolytica in Biotechnological Applications

The nonconventional yeast Yarrowia lipolytica has been developed as a versatile and attractive tool for a large variety of biotechnological applications. This yeast has several physiological properties with industrial significance. Y. lipolytica uses hydrophobic substrates such as n-alkanes, oils, fats, and fatty acids as low-cost carbon sources. The yeast is able to produce a set of diverse ad...

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ژورنال

عنوان ژورنال: Microbial Cell Factories

سال: 2017

ISSN: 1475-2859

DOI: 10.1186/s12934-017-0755-0